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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which leads to chromosome instability, and thus may perhaps contribute to tumorigenesis. This protein was uncovered to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a essential role in advertising mobile-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are actually described. Two added splice variants had been documented but in depth nucleotide sequence details will not be still available.
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Blocking peptides are peptides that bind specially to the concentrate on antibody and block antibody binding. These peptide commonly incorporates the epitope identified via the antibody.
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This guidebook outlines additional feasible optimization of mobile and immunoassay parameters to guarantee the absolute best effects are...
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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to be sure the absolute best effects are...
Antibodies sure to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, one can see which staining is unique.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic event. Cyclin E sorts a posh with and functions being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates in the G1-S stage boundary and is degraded as cells progress by way of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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